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With proper purification and fractionation techniques, the contents of a particular fraction will correspond to a particular organelle.
Hence, presence of mullite in higher density fraction indicates the higher concentration of mineral matter in that particular fraction.
Each point of the ROC curve then represents a unique TPF/FPF pair corresponding to a particular fraction of the molecular dataset.
To investigate these particular fraction of proteolytical activity of the human degradome even in a complex cellular environment, chemical probes that covalently label the corresponding proteases proved to be versatile tools.
When examining Fig. 4, it may seem counterintuitive that for radii r = 2 (Fig. 4c) and r = 3 (Fig. 4d) a particular fraction of Compound has more unique atom environments than Substance; however, there is no direct correlation between the percentage of Substance and Compound, per se, due to the duplicity and order of chemical structures in Substance.
All peptide ratios for a specific protein present in a particular fraction were then normalized and log-averaged to obtain the local relative protein ratio.
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The wound healing capacity in presence of the control-conditioned medium (absence of K. oxytoca) was compared to the control-conditioned medium in which particular fractions (x > 10 kDa; 10 kDa > x > 3 kDa) were interchanged with the same fraction of K. oxytoca-conditioned medium.
Equal volumes of particular fractions were analyzed by SDS-PAGE and western blotting.
For direct Western blot analysis, cell lysates or the particular fractions containing 50 µg protein was separated by SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane.
A potential inherent weakness in this type of studies, however, is that neurosphere culture studies only assess the particular fractions of glioma cells, which are supported by the neurosphere culture conditions [10], and the neurosphere culture conditions do not necessarily represent the glioma niche environment.
The quality of separation of particular fractions was verified microscopically.
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