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This was identified by visually inspecting the disappearance of bands, as was as the point the binding curve for the diglycosylated PrPsc band intersected the background density of the particular blot.
The results in Figure 4A showed that each of these mutants contain mostly normal-length ATG31 transcripts; however, low levels of readthrough are apparent in some strains, most obvious in nhp6 (lane 3) and smc3 (lane 12) mutants in the particular blot shown.
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In particular, blotting of neoplastic tissues produced two different types of band, either of weak or strong intensity.
In particular, Western blot analysis showed an increase in the phosphorylation of both kinases after exosome treatment.
In particular, western blot analysis for Bid and tBid detection in HeLa cells and Neuro2A cells disclosed that mutated htt transfection causes Bid cleavage.
In particular, northern blot analysis suggested that our strategy allowed us to identify some genes that may be specifically induced at high levels in Cx whereas are not expressed or expressed at very low levels in Ox.
Depending on the strength of the probe, the particular digests blotted on the membrane, and the background of the detection, some blots are left to develop for as long as 2 h, while others require less than 1 h.
In particular, western blotting revealed the presence of a band corresponding to the full-length FIX form (∼60 kDa), which migrated as plasma-derived FIX.
In particular, the Western blot derived from E1 antibody showed that VP1-related proteins had extensive cross-linking (Fig. 5B, lane 2), and most likely the pentamer was covalently cross-linked together because no protein bands below 200 kDa MW were observed.
As only a small amount of tissue is obtained from a murine brain, biochemical methods, in particular a western blotting technique for PrPSc had to be adapted to maximise sensitivity.
In particular, both northern blotting and in organello transcription analyses showed that one compensatory key point is at the level of transcription, since the ratio between mtDNA transcripts and mtDNA content in Mpv17−/− organelles was several fold higher than in Mpv17+/+ organelles.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com