Sentence examples for particles were eluted from inspiring English sources

After loading of the lentiviral particles to the MA and washing, retained viral particles were eluted with high salt elution buffer (1 M sodium chloride).

After washing the column with 10 bed volumes of binding buffer the viral particles were eluted with 5 bed volumes of elution buffer (10 mM Na2PO4, 1 M NaCl, pH 7) and then concentrated using a spin column with a molecular weight cut-off (MWCO) of 30 kDa (Vivaspin, Vivascience, Germany; cat. no. VS1521).

After three weeks, 29, 74 and 81 phage particles were eluted, respectively.

The bound phage particles were eluted by the addition of sodium acetate buffer (100 µl; 0.1M acetic acid, 0.15M NaCl, pH = 2.8) and incubation for 8 minutes followed by neutralization with 12 µl 2M Tris buffer, pH = 9.5.

Finally, AAV particles were eluted in sterile PBS and stored at − 80 °C until use.

Protein fractions containing phage particles were eluted early during the run due to their high molecular mass.

For such large particles AsFlFFF works in the steric or hyperlayer mode, with the largest particles being eluted first.

Proteins were eluted from the washed particles by means of two subsequent elution steps with 60% acetonitrile and 2% acetic acid.

Proteins were eluted from the magnetic particles by addition of 40% methanol containing 0.1% formic acid.

Particles of approximately 20 40 nm in diameter were eluted from ligand-coated beads from both R6/2 and HdhQ150/Q150 brains at all ages tested (Fig.  5A).

After extensive washing to remove non-specific binding, repeat-containing DNA fragments were eluted employing a magnetic particle concentrator.