To determine infectivity, which we defined as transducing units per physical particle, we designed an ad hoc quantitative polymerase chain reaction (PCR) that selectively amplified the reverse-transcribed vector genome and discriminated it from plasmid DNA, which was carried over from the transfection used to produce the vectors (Supporting Information Fig. 1).
For the purpose of proteins bio-conjugation to Gold Nano-Particles (GNPs), we designed and synthesized a polypeptide named NanoLock, derived from SNARE (Soluble NSF Attachment protein REceptor) proteins and able to form a remarkably stable complex with the SNARE protein SNAP25 (Synaptosome Associated Protein of 25 kDa).
In an attempt to study the effect of various radiation sensitive groups in the polymer matrix and their relation to the sensitivity towards charged particles, we have designed two new monomers Allyl bis- 2-nitroxy-ethyl) carbis- 2-nitroxy-ethylrepared its copolymer with ADC.
In contrast to polycationic micelles reported so far, we designed particles integrating a sufficient nucleic acid condensation capability by polycationic polyethylenimine (PEI) segments as well as only a mild cytotoxic behavior.
Furthermore, we designed particles that contain boronic acid groups, which are known to form complexes with diol groups of target biomolecules.
Patton explains: "We designed the particles so that when they hit the lungs, they dissolve almost immediately.
In this work, we designed replica particles based on poly (l-lysine) (PLL) polymers crosslinked via a homobifunctional linker to support coadsorption of a plasmid DNA and a peptide hormone for concurrent transfection and induction of a cellular function.
We designed a chaotic binary particle swarm optimization (CBPSO) algorithm to search potential syndromes for diagnosing efficiently.
Via the use of a mesoscopic simulation technique called dissipative particle dynamics, we design sterically stable biocompatible vehicles through the self-assembly of a binary mixture composed of amphiphilic molecular species, such as PEGylated lipids, and phospholipids.
Shape-controlled particles were designed to have two non-centrosymmetric oriented functionalities.
