In total, >11 000 images of individual particles, selected from 287 micrographs, were used for the analysis (Supplementary Figure S2).
After manual verification, 90 894 particles were selected for single-particle classification and reconstruction, following standard SPIDER protocols for reference-based reconstruction (LeBarron et al, 2008; Shaikh et al, 2008).
The average number of charges on a 990 nm PSL as measured by ICD2 for particles selected with the QD set to 400 V is 17000.
These coarse particles are selected for their high resistance to being displaced by strong currents and thus protect the underlying finer sand particles from scour.
Four parameters, including SiO2 mass percentage, type of alcohol, sieved size of SiO2, and sieved size of Ca(OH 2 particles, were selected for conducting experiments in a fluidized bed.
The three flows, consisted of various constitute particles, were selected for comparison.
Five characteristic regions of different distribution of the MNCM particles were selected for the further study (see Fig. 1c, d).
5KC cells were transduced with the viral particles and selected for stable lines with puromycin according to the manufacturer's instructions.
Small ring-like particles were selected for statistical analysis to determine if they may represent the end-views of short hDmc1-ssDNA filaments (Figure 1A, yellow circles).
For these reasons, osteochondral scaffolds with pillar BCP particles were selected for in vivo studies and seeded with MSCs, known to be able to differentiate into musculoskeletal tissues under the appropriate stimuli.
