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The present study was designed to determine the effect of nano copper particles on cell injury of intestinal epithelial cells (IECs) in piglets.
For determining the effect of particles on cell viability, different assays were used.
Furthermore, we quantified the effect of pSi4.1-siFAK phagemid particles on cell invasion.
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The reliability of these models depends on the introduction and deposition of aerosolized particles on cells at an air liquid interface.
SEM was used to confirm attachment of particles on cells and observe the orientation of attachment of particles of different shapes.
In no case has the actual uptake of amorphous silica actually been examined and correlated with the effects of the particles on cells; the flow cytometry assays used in the past cannot distinguish between surface-bound particles and internalized particles.
There are several approaches to evaluate the toxic effects of particles on cells that have been suggested or pointed as targets of PM and NP.
The literature on the toxic effect of amine-modified particles on cells highlights lipid membrane disruption as a key determinant, and our TEM micrographs (see the Supporting Information) confirm significant membrane damage at 3 and 24 h following PEI-QD exposure.
As shown in Figure 8C (and Figure 1), 12 hours after infection we no longer detected viral particles on the cell surface in untreated cells reflecting complete uptake of PsVs (Figure 8C, control siRNA, L1 K75).
This study showed that scFv phage are applicable for a broad range of anti-VEEV diagnosis assays: antigen ELISA on purified virus particles, ELISA on cell lysate and immunoblot.
Recently, it was suggested for direct toxicity mechanisms of nanoparticles to induce a direct alteration of cellular exchanges with the media due to particles binding on cell membrane [ 21].
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