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Co-localizing puncta were evaluated using the Analyze Particles function in ImageJ.
Positively stained cells were identified and counted using the analyse particles function in Image J software (rsbweb.nih.gov).
After applying this function, the number of highlighted Fos immunoreactive nuclei were counted using the analyze particles function in ImageJ.
Measurements are then provided of selected areas using the "analyse particles" function in ImageJ (Fig. 3 a and b).
The number and area of peripheral focal adhesions per cell was then determined with the "Analyze Particles" function in ImageJ (NIH).
Each cell in the image was outlined, and the outlined area was analyzed for mitochondrial number, average size and total area using the Analyze Particles function in ImageJ software (NIH).
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Thresholded GluA1 punctal size and density in dendritic shafts was analyzed using the Analyze Particle function in ImageJ.
The 'analyze particle' function in NIH ImageJ was used to perform the measurement and data was exported to MS-Excel for analyses.
Previous works (Zhao et al. 2015; Zhou et al. 2015) showed that human 20S particle functioning in membrane fusion processes in eukaryotic cells is composed of two parts relatively flexible to each other: the SS complex with pseudo four-fold symmetry and the hexameric NSF complex.
The mean intensity of the resulting signal within the ROI was calculated with the built-in Analyse Particles function.
Then the signals outside SUR1 ABC transporter module were subtracted from each particles using particle subtraction function in Relion 2.0 as described previously (Bai et al., 2015).
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