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Exact(28)
Colloid that was not aged resulted in globular, ill-defined particles, indicating the importance of sulfide in determining the overall shape of the particles (data not shown).
Similar results were obtained with latex particles (data not shown) and with RAW264.7 macrophage cells (Fig. S2).
Both of these media showed similar IgM-mediated binding and uptake of small particles (data not shown).
Coating had no effect on either the rate or the extent of internalization of particles (data not shown).
The uninfected cells, in contrast, did not have noticeable DAB staining or immuno-gold particles (data not shown).
Phage lysates treated with DNase I showed the same results as untreated phage particles (data not shown).
Similar(32)
The intensity profile taken along the diameter of any of the three round shapes fits well with the response expected from a 1.2 μm sized particle (data not shown).
We did not find any relationship between SIDS and PM10 or the coarse particles (PM10 PM2.5) (data not shown).
Leukocytes other than monocytes do not accumulate the particle significantly (data not shown).
The labeling is antibody-specific because incubation of non-derivatized fluorescent CLIO for the same duration and temperature does not result in significant particle uptake (data not shown).
Cells infected with a specific inhibitor of endocytosis (a dominant negative K44A dynamin mutant) or treated with pharmacologic endocytosis inhibitors (phenylarsine oxide, nystatin, methyl-β-cyclodextrin) still lost considerable β1 integrin after NeoA treatment (Figure 9A) and they continued to release NeoA-induced vesicles (assessed with a Nicomp particle sizer; data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com