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The idle ribosome-Sec61 map was obtained using the 69,464 particles (Classes 1-3 and 5-10) that did not contain tRNAs or a nascent peptide, refined using a 60S mask to 3.4 Å resolution.
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Moreover, the application of the Eu La approach is essential when multiple particle classes are present.
Generic geometric shapes representing different types of crystals are defined as particle classes.
Therefore, for consideration of different particle classes, the Eu La approach is much cheaper than the Eu Eu approach.
Hallanger et al. (1996) utilized a secondary water particle distribution with an average diameter of 250 μm and a Rosin Rammler exponent of 3 for seven different particle classes.
The fall time threshold allows the model to distinguish between dense (lithic) and light (pumice) particles, however for simplicity the density of both particle classes is set to the median density of 1950 kg m-3.
Nevertheless, prediction of modeling uncertainties is challenging and the computational costs for solving the additional transport equations are quite high, particularly if multiple particle classes are present in the flow.
Physical properties of the particles, the specific surface (BET), pore volume and pore volume distribution over pore sizes (BJH) were determined for all particle classes by N2 adsorption/desorption experiments.
The percent changes were not significantly different between colesevelam and placebo for the other particle classes.
(C ) A montage of representative particle classes of the TorsinA(E171Q):LULL1 complex obtained from negatively stained particles are shown.
New reconstructions were calculated after class assignment, and the procedure was repeated for another 20 cycles, at which point the particle classes remained essentially unchanged.
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