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K562 cells were transduced separately with empty shRNA vector control, three different ME2 and one ACL shRNA lentiviral particles as previously described [59].
Plasmid DNA was precipitated onto 1 3 µm gold particles as previously described [40] at a rate of 1.8 µg pPML7800 and 0.2 µg pPML7842 (10∶1 ratio of DNA vaccine to adjuvant) per 1.0 mg of gold.
Vectors were packaged into viral particles as previously described.
This area hosted often times birefringent particles, as previously described (11).
AMCV viral cDNA was retrotranscribed from genomic RNA extracted from purified virus particles as previously described [ 25].
To verify that antibodies against mimivirus in patients with pneumonia recognize mimivirus particles specifically, a serum sample of 1 of these patients was used to detect mimivirus particles as previously described (12 ).
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Surprisingly, V is not uniformly distributed in the particle as previously observed.
To achieve this, we labeled the myosin head with a 20 nm gold particle as previously and the tail with a GFP booster (Ries et al., 2012).
Formulation of these DNA-PLG particles was as previously described [50].
The mutated P proteins were expressed and purified by E. coli system (BL21) as P particles as described previously (Tan et al., 2008; Tan and Jiang, 2005; Tan et al., 2009).
VP4 formed tubule-like particles) as reported previously [ 21, 27].
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