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Immunogold labeling was performed using an anti-rRNA antibody followed by anti-mouse IgG coupled with 10 nm of gold particles, and samples were viewed using a Tecnai Spirit transmission electron microscope (FEI) at 80 kV.
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It is known from the sample preparation that all processing for both samples, i.e., PSt/TiO2 composite particles and sample D, was conducted at 30°C or lower prior to this TGA test, which means that there is moisture remaining in the samples.
Such contradictory results make it difficult to choose the optimal conditions, and we suggest testing more contenders for non-specific binding in order to discover the optimal conditions for particular TiO2 particles and sample.
This probe could be employed to scan surface topographies by mapping f-d curves, and the interaction force between the charged Teflon particle and sample would give a direct indication of the local electric field and properties of the sample.
The list of minerals used in this study was chosen based on the mineralogy of meteorites, interplanetary dust particles and Stardust samples.
Differential scanning calorimetry reveals that polymer crystals nucleate heterogeneously on graphite particles and that samples containing graphite have higher degrees of crystallinity than the neat polymer.
In the present work, six monoclonal antibodies (mAbs, two for each virus) specific for conserved proteins from hRSV, hMPV and ADV were generated and evaluated through different immunological techniques, based on detection of purified protein, viral particles and human samples.
Results show that there was no significant difference among the control (without adding alginate particles) and the samples.
For thin-sections, washed particles and NB samples were dehydrated with two washes of 100% ethanol.
Aliquots of washed particles and NB samples were processed for micro-Raman spectroscopy as described before [2].
For transmission electron microscopy (TEM), washed particles and NB samples were deposited on formvar carbon-coated grids and were dried overnight as described above.
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