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Bacterial clones were partially sequenced with the primer 27F.
Bacterial clones were partially sequenced with use of the primer 27F.
The sequencing reactions were performed using two primers specific to the trnD- trnT and ITS markers, and the trnH- trnK locus was partially sequenced with primer H, as performed by Nettel and colleagues [ 23].
Given the high level of genome sequence similarity and the fact that the genomes of strains ZJU1202 and DZ2Q have only been partially sequenced with numerous gaps, which hinders the accurate comparison, we believe that the common genes shared by these three strains could be substantially higher than the currently obtained 3502.
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The predominant band at 15 kDa in the turtle eye was partially sequenced de novo with mass spectrometric methods.
We also compared the Prunus BAC sequences with the partially sequenced Medicago genome to gain further insight on the genome evolution of the related plant species.
PCR products of the partially sequenced loci were visualized with GelRed (Biotium) on a 1.5% agarose gel and sequenced on a 3130 xl capillary sequencer (Applied Biosystems).
The challenge is bigger using polyploid species with partially sequenced and assembled genome such as the hexaploid wheat having a high content of repetitive DNA.
We propose that CSRBH should be the method of choice for researchers working with non-model species which have no sequenced genome and partially sequenced genes.
In the analysis with the partially sequenced Medicago genome, we found one syntenic region that shares four genes with the peach BAC PpN089G02.
The other ST1 genome from strain GZ1 was compared with a partially sequenced ST25 genome (89 1591) available at the time revealing 132 genomic islands including 5 pathogenicity islands [20].
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