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Eighteen fractions were collected, partially pooled and applied to a C18 nano-column on a Bruker maxis 4G ETD QTOF LC-MS instrument, and separated with a 5 32 % acetonitrile gradient with 0.1 % formic acid (followed by a 32 80 % gradient to elute large peptides).
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MCF-7 cells were treated with the partially purified pooled fractions and subjected to the MTT assay to determine their effect on cell proliferation.
All those cases in which at least one segment was partially fused (P) were pooled into the 'partially fused' category.
Populations were pooled by partially thawing glycerol stocks until 100 μL could be removed, which was inoculated into 5 mL LB and grown to stationary phase.
Commercial ELISA with recombinant antigens studied in phase three investigations partially explained heterogeneity, and pooled sensitivity and specificity were 99.3%[97.9%-99.9[97.9%-99.9%% [88.5%-99.5%] respectively.
Live males and partially fed females were pooled by species and held at 25°C and a relative humidity of 98% until they were allowed to reattach and feed on uninfected horses.
The partially purified protein fractions were pooled and qualitatively analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), Voyager-De-STR (Applied Biosystems, Framingham, MA, USA).
However, partially filled pool of heavy liquid metal poses safety concerns related to seismic loads.
The house, built around 1900 and thoroughly renovated 10 years ago, is 1,631 square feet and has vaulted ceilings, an office, a 30-foot partially covered pool, two and a half bathrooms and a recently added artist's studio.
One interpretation is that the central manual tracking task and the peripheral target detection task tapped into partially independent pools of information-processing resources (Wickens, 2002).
We subjected the partially purified pool of free untagged Brk1 to glutaraldehyde cross-linking (Fig. 3C).
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