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In contrast, in cells exclusively expressing A3ZFm2-myc the fully edited A6 mRNA was essentially absent while partially edited and pre-edited A6 mRNA accumulated; edited MURF2 mRNA was similarly diminished and partially edited and pre-edited MURF2 mRNA accumulated, despite the amount of KREPA3ZFm2-myc being similar to that of untagged KREPA3.
Exclusive expression of A3ZFm2-myc also resulted in the loss of edited MURF2 mRNA and the accumulation of pre-edited and partially edited MURF2 mRNA, although this is less obvious since the region of MURF2 that is edited is much smaller than that of A6.
Detailed analysis of edited RNA products revealed the accumulation of partially edited mRNAs with less insertion editing compared to the partially edited mRNAs found in the cells with wild type KREPA3 expression.
Overall, 10 (9.1%) and 18 (17%) fully edited sites were present in leaf and floral tissue, respectively; 26 (23.6%) and 30 (28.3%) high partially edited sites; 15 (13.6%) and 11 (10.4%) medium partially edited sites; 14 (12.7%) and 6 (5.7%) low partially edited sites; and 45 (40.9%) and 41 (39.6%) poor partially edited sites (Additional file 1: Figure S1, Table S2).
To determine if the in vivo editing deficiency observed in partially edited A6 sequences was also present in other edited RNAs, MURF2 in vivo editing was assessed in RKO-A3ZFm2-myc cells.
For three out of the four partially edited sites in C. reflexa and for one partial editing site in C. gronovii, we could observe higher editing efficiencies in photosynthetic active tissue (in the tips of the seedlings of C. reflexa and C. gronovii grown without a host plant; Fig. 4).
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One of latter is edited partially (psbJeU60LL) (table 2).
The moss Takakia lepidozioides has been partially characterized for editing sites, and 302 C-to-U and no U-to-C editing sites were reported (Yura et al. 2008).
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