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pTMVΔcp was then generated from pTMV-KXEN by partially deleting the CP gene from downstream +55 bp of the start codon of CP.
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This mutation is a 324bp deletion that partially deletes exons 7 and 8, resulting in a premature stop at amino acid 432.
Mapping of the rearrangement breakpoints by inverse-PCR revealed that the deletion spans four genes: Fam162a (partially deleted), Ccdc58 (completely deleted), Csta (completely deleted), and Stfa2l1 (completely deleted) [1].
Our study demonstrates that deleting MHR1 has a strong impact on mtDNA maintenance, with the formation of partially deleted mitochondrial genomes due to large deletions.
The C-terminal construct contains a large portion of the predicted myosin-tail domain of CEP290 that has been hypothesized to play a vision-specific role as this region is partially deleted in the rd16 mouse model of retinal degeneration (deletion of amino acids 1599 1897) (4).
As shown in Figure 8B, deletion of RAD9, RAD24, MRC1, CHK1, DUN1 or PDS1 (partially deleted as a temperature sensitive mutant, see Materials and Methods) promoted the proliferation of type I cells on plates containing bleomycin.
Analysis of genetic recombination between two partially deleted lactose operons of Escherichia coli K-12.
The cagPAI positivity included both intact and partially deleted, with the overall frequencies of 70.5% and 26.2%, respectively.
Finally, our study proposed that H. pylori strains with partially deleted cagPAI were less likely to cause severe diseases in comparison with those carrying intact cagPAI.
Here, we studied lentiviral vectors containing either the full-length 1.2-kb insulator or the smaller 0.25-kb core element in both orientations in the partially deleted long-terminal repeat.
Genotypes in this group contain two genes (norB and cypA) on the left end and lost 13 genes leaving partially deleted verA at the middle of the cluster.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com