Diazotroph rhizosphere community was characterized at 21 days in terms of size (quantitative PCR of nifH genes), composition (T-RFLP and partial sequencing of nifH alleles) and functioning (quantitative RT-PCR, T-RFLP and partial sequencing of nifH transcripts).
The diazotroph community was characterized at 21 days in terms of size (nifH quantitative PCR), composition (nifH T-RFLP and partial sequencing of nifH gene) and functioning (quantitative RT-PCR, T-RFLP and partial sequencing of nifH transcripts).
Partial sequencing of genomic DNA demonstrated the presence of three introns in the coding DNA.
The performance of the database was then tested on 124 clinical and 16 environmental isolates previously characterized by partial sequencing of the β‐tubulin and calmodulin genes.
Partial sequencing of the PCR products gave a translated sequence 100% identical with the amino acid sequence of P. aeruginosa amidase.
Partial sequencing of CL-1 has confirmed the presence of sequences which code for calmodulin.
The bacterial isolates were further characterized, using the partial sequencing of 16S rRNA gene.
Screening of the clone library, partial sequencing of representative clones and sequence analysis were performed as described by Ziganshin et al. [9].
The results of partial sequencing of cloned 16 S rRNA amplicons and the corresponding T-RF values are listed in Additional file 1.
The identity of all fungi involved was confirmed by partial sequencing of 18S rDNA (not reported).
Partial sequencing of the NS5B region was performed in 310 isolates circulating in patients from 1995 to 2007.
