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The bacterial isolates were further characterized, using the partial sequencing of 16S rRNA gene.
Both strains were identified to be K. ohmeri upon partial sequencing.
Partial sequencing of genomic DNA demonstrated the presence of three introns in the coding DNA.
Screening of the clone library, partial sequencing of representative clones and sequence analysis were performed as described by Ziganshin et al. [9].
The results of partial sequencing of cloned 16 S rRNA amplicons and the corresponding T-RF values are listed in Additional file 1.
The performance of the database was then tested on 124 clinical and 16 environmental isolates previously characterized by partial sequencing of the β‐tubulin and calmodulin genes.
Partial sequencing of the PCR products gave a translated sequence 100% identical with the amino acid sequence of P. aeruginosa amidase.
Deletion of rrnB was confirmed by Southern blot analysis and partial sequencing.
Partial sequencing allowed the inclusion of ovorubin among the small Kunitz-type inhibitors family.
The identity of all fungi involved was confirmed by partial sequencing of 18S rDNA (not reported).
Partial sequencing of the NS5B region was performed in 310 isolates circulating in patients from 1995 to 2007.
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