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The model could then be used to predict growth behaviour with full medium exchange, but showed some reduced predictive ability after partial medium exchange.
Partial medium changes were performed every 5 days.
Partial medium changes with fresh CM were performed every 3 days.
hES cell culture medium for bFGF-hFLSCs feeders was also identical to the medium used in MEFs feeders but removal of the bFGF, and the medium were changed daily by partial medium changes.
Starting at day 7, anti-TSP-1 (1∶1000 Calbiochem, Gibbstown, NJ) was added to the cultures, and fresh anti-TSP-1 was replenished with partial medium changes every 3 days.
Homogeneous cultures of erythroid progenitors established after several days were kept in the same medium at 2 × 10 cells/mL by daily partial medium changes.
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The photophysical behavior of probe and its affinity toward different metal ions have been investigated in partial aqueous medium, protein medium and live cells.
The photophysical behavior of the probe 7 has been examined and tested for the recognition of ions in partial aqueous medium and HeLa cell.
As a proof of concept and promising prospects for application in biological sciences probe 1 has been utilized to detect ATP sensitively in a partial aqueous medium and intracellularly in HeLa cells.
The multiple sequencing analysis of the new 66 full-length OROV N (small RNA) and for the 36 partial Gn (medium RNA) and large RNA gene sequences showed high nucleotide and amino acid identities (>90%).
Stagnant flooding (prolonged partial flooding; medium deep) occurs in areas when floodwater of 25 50 cm stagnates in the field from a few weeks to several months (Khush 1984; Singh et al. 2011).
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