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We checked the density distributions of each mutant reconstruction for signs of partial decoration or mixed populations, which would change the relative strength of features in the maps.
Partial decoration would be immediately apparent in the images by disruptions of the characteristic arrow-head appearance of myosin-decorated actin filaments.
Because AVID measures the variance between asymmetric units, partial decoration leads to an AVID map where there is no density in the area of the filament being decorated but clear density in the area where partial occupancy occurs.
Thus, the differences between 3D reconstructions for actin filaments decorated with wild-type smooth muscle S1 and those containing the R403Q mutation are solely due to changes in variability in the entire myosin region and not due to partial decoration.
Again, these new reconstructions showed the same relative change in the strength of the features in the maps, reinforcing the notion that this effect is not due to partial decoration.
To ensure that this effect is not caused by partial decoration of the filaments (as opposed to a true mix of conformations), we carefully analyzed the appearance of the filaments before we selected them for averaging.
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Maurice Boffa considered a stronger axiom: every partial, injective decoration of an extensional set graph G whose domain contains the G-preimages of all its elements can be extended to an injective decoration of all of G.
Decorate!" cookie decoration.
The Pompeian Villa was almost completely restored after the earthquake of 62 A.D. Successive renovations caused the partial destruction of earlier room decorations of the fourth, third, and second styles, and the discarded material was used to raise the floor level of the garden.
In their pristine form, C60 NRs suffer from partial damage in the solution-based decoration process resulting in poor crystallinity.
In his book The Small House, tiny-house builder, dweller, and pioneer Jay Shafer presents the tiny house as an antidote to American McMansions our "bloated warehouses full of toys, furniture, and decorations"—and as a partial solution to energy overconsumption.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com