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Almost every SOFAR member has a corresponding protein, except in the case of partial coding sequences, predicted genes and non-coding RNAs.

Among these contigs, 55.5% covered the full coding sequences of a gene including partial sequences of 5' and 3' untranslated regions (UTRs), 26.2% partially covered coding sequences with partial 3' UTR, 8.1% covered partial coding sequences with partial 5' UTR, and 10.2% only contained partial coding sequences.

The partial coding sequence of LhSorTGA2 was retrieved from sequences annotated in our previous RNA-seq data.

The primer pairs, NHCo1/NHCo2 and NHFe1/NHFe2 yield PCR products corresponding to a partial coding sequence of cobalt and iron NHase genes, respectively.

The comparison of the identified partial coding sequence of U. tumidus with those available from vertebrates and invertebrates indicated that GCL sequence was highly conserved.

The cDNA full-length sequence of LhSorTGA2 was obtained by assembling the 5′-RACE sequence, the partial coding sequence, and the 3′-RACE sequence.

In the present study, the partial coding sequence of LhSorTGA2 was retrieved from our previous transcriptome sequencing data, and the LhSorTGA2 full-length cDNA was obtained by RACE.

The full-length sequence was obtained by assembling the 5′-RACE sequence, the partial coding sequence, and the 3′-RACE sequence.

Second, we combined control and partial coding region information known to have different mutation rates.

On the other hand, sequence lengths are long enough to suggest identification of full or partial coding sequences.

As a control, we used a tandem vector containing partial coding regions of red fluorescent protein (dsred).

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