Exact(4)
On the contrary, a well conserved divergent 3'-untranslated part sequence led to a common SAGE tag identified in zp2.3 and zp2.4 transcripts, while a distinct SAGE tag was recovered with zp2.2 (Tables 1 and 5).
The target gene to be silenced can include a single gene or part sequence of a single gene that is targeted for suppression or can include multiple consecutive segments of a target gene, multiple nonconsecutive segments of a target gene, multiple alleles of a target gene, or multiple target genes from one or more species.
Also, as shown in Figure 2A, both the 3'-end COLD1_10_H04.b1_A029, displayed as a reverse complement view, and its 5'-end counter part sequence, COLD1_10_H04.g1_A029, contain the "double-termini adapters" structure to join two different transcripts and form a chimeric cDNA insert.
Semi-quantitative RT-PCR was performed using a forward primer containing the 5' part sequence of miRNA and a universal primer complementary to the stem-loop part of RT primer at 94°C for 2 min, followed by 21 cycles of 94°C for 15 s and 60°C for 1 min. The reaction products (about 60 bp) were analyzed by electrophoresis on a 2.5% agarose gel in 1× TAE.
Similar(56)
The proposed GA-based heuristic determines the part-type sequence and the operation-machine allocation that guarantee the optimal solution to the problem, rather than using fixed, predetermined part sequencing rules.
Consequently, for the most part, sequences of expressed sequence tags (ESTs) were acquired.
Supporting Information Table 3 contains all of the part sequences used in this study.
The phylogenetic analyses were carried out with the middle part sequences of HA and NA genes.
However, corresponding differentially expressed transcripts were discriminated by a specific SAGE tag, due to divergent 3'-untranslated part sequences.
In many instances, PSI-BLAST detected pART sequences from distantly related organisms in earlier iterations than the human pART paralogues from other subgroups.
Spades is the first of a four-part sequence of plays that Lepage is developing for a network of 13 theatre-in-the-round venues, from Canada to Croatia.
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