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While G482T is proximal to various miRNA-binding sequences, it is not part of the seed sequence for any of them.
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Interestingly, within all these five seed groups, the location of the seed sequence alternates between 5′ and 3′.
The last column gives the orthologues of the seed sequences.
However, we found that the seed sequence of BART9-3p identicalcal to the seed sequence of the human miR-200 famincludinguding miR-200a and miR-141.
We also considered the seed sequence (positions 2 7) separately from other parts of the mature sequence because the seed sequence is known to be most critical for target recognition (see Bartel 2009 for review).
The miRNA sequence 3′ to the seed sequence has variable degrees of complementarity with the mRNA.
A third filtering step required the presence of at least some part of the miR-seed sequence, nucleotides 2 7.
The seed sequences of each of the miRNAs were defined based on criteria described in [41].
We therefore compared the seed sequences of all mature BART miRNAs to the seed sequences of all known human miRNAs (Lewis et al. 2005).
All these facts point to a possible functional role for the seed sequences, similar to those of miRNA seeds.
Seed testing is thus an important part of the seed industry.
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