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This was followed by an abrupt interruption in the coverage where the drug selection cassette hdhfr was integrated and a drastic reduction in the downstream part of the coding sequence (Fig. 2c), indicating that a transcript encompassing the AP2 DNA-binding domain was overexpressed in the AP2 mutant parasites.
Polymerase chain reaction (PCR) amplifications were performed using combinations of a set of primers spanning a large part of the coding sequence, listed in Table S1.
Different types of prediction error were considered, such as excluding coding exons, including introns as part of the coding sequence, or wrongly predicting start and termination sites.
To corroborate the dominant negative experiments, we analyzed the effects on spine morphology of two different TRIM3 RNAi constructs (TRIM3/264, directed against part of the coding sequence, and TRIM3/2756, targeted against the 3' untranslated region).
In the CACTAs, two copies of the Cactavine-5 family contain part of the coding sequence and the 3' untranslated region of a gene encoding for an unknown protein that contains a pentatricopeptide repeat (PPR) domain (Figure 5).
Then, they were purified, restricted with appropriate endonucleases and ligated into the pMAD vector in order to generate truncated allele of the gene of interest where the most part of the coding sequence (CDS) was deleted (60 to 80% of the CDS corresponding to the median part of the gene).
Similar(39)
The tfs3 of strain B8 has one coding sequence less (HPP12_ virB-2) and a merged coding sequence HPB8_543 of which the first part corresponds to the coding sequence HPP12_1331 and the second part to the coding sequence HPP12_1332.
We used 2 lentiviral shRNAs targeting different parts of the coding sequence of BIRC6.
Even with new proteins, mostly several EST that contain parts of the coding sequence can be found.
Silencing of BIRC6 with two shRNAs targeting different parts of the coding sequence resulted in increased cytoplasmic DIABLO levels and triggered apoptosis.
First, we identified variation in Xpr1 alleles by sequencing parts of the coding sequence expected to be variable (Additional file 1: Figure S3), i.e. exon 4 and two putative extracellular loops (ECL3 and 4).
More suggestions(15)
part of the target sequence
part of the accident sequence
part of the hallmark sequence
part of the analysis sequence
part of the adaptor sequence
part of the chromosome sequence
part of the seed sequence
part of the probe sequence
part of the coding process
part of the coding region
part of the testing sequence
part of the motor sequence
part of the processing sequence
part of the loading sequence
part of the development sequence
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