As we have seen, the combination of mutation extraction tools recover a large part of the mutations curated in existing databases, and therefore combining several tools together is a viable strategy for genetic variant extraction, but there are still variants that are not covered.
However, this theory of sexual antagonism rests on the assumption that a significant part of these mutations is dominant [ 18], as recessive mutations beneficial to males would accumulate on the X chromosome.
As eukaryotic genomes contain a large number of random mutations, it is thus reasonable to expect that part of these mutations may lead to 'intrinsically insoluble proteins'.
Non-random mating and inbreeding, when outcrossing rates are low to absent, are able to increase the frequency of homozygous genotypes and therefore eliminate large part of deleterious mutations that are not completely dominant.
Because barcode tags are part of the mutations, the proportion of an individual barcode reflects the proportion of that barcode-associated mutant in a population.
This implies as well that a small part of the mutations in the databases are not the fully compliant with the HGVS nomenclature.
Phase 2 actually is a phase of adaptation to a new host for the majority of the lineages and based on the phenotypic changes occurring during this phase, we can be sure that at least part of the mutations acquired was not neutral.
As part of the mutation library development for TILLING, mutations are induced throughout the genome and these additional background mutations are carried along in crosses that combine mutations in the target gene of interest.
GD, EA, BP, CT, DM, CB and CDD performed part of the mutation analysis and interpretation of the data.
SK participated in the design of the study, performed and evaluated the immunohistochemistry, performed the PCR part of the mutation analysis, and wrote the paper.
