Sentence examples for parp detection from inspiring English sources
Apoptosis was also assessed by the determination of PARP and caspase 8 cleavage by western blotting using anti-mouse caspase-8 from R&D and rabbit PARP (detection of the short cleaved form) or mouse PARP (detection of the large fragment) antibodies (Santa Cruz).
In addition, in MDA-MB-453 cells, which are intrinsically resistant to Lapatinib treatment, Lapatinib did not enhance levels of Bim or apoptosis, as assessed by cleaved PARP detection (Fig. 1c).
We also showed at 72 h that extinction of Scd1 activity by siRNA and inhibitors (data not shown) induced apoptosis in HCT116 evidenced by active caspase 3 or PARP-cleavage detection (Figures 5B and 5C).
PARP is required for detection and signaling of single-strand break repair (SSBR).
In these experiments, cell shedding was confirmed to be due to apoptosis by acridine orange staining for apoptotic morphology and by detection of PARP cleavage (data not shown).
This is evident by microscopic examination (data not shown) and detection of PARP cleavage, a well-described indicator of effector caspase activation and consequent cell death.
Twenty-four hours later, apoptosis was determined by caspase 3/7 activation and by the detection of PARP cleavage that serves as a marker of cells undergoing apoptosis.
Apoptosis induction upon in vitro EL102 and docetaxel administration was further evidenced by detection of PARP cleavage in protein extracted from DU145 cell lysate, 24 and 48 h post-treatment.
Annexin V-FITC/PI staining by FCM analysis, TUNEL assay and the detection of PARP and cleaved caspase3 expression levels demonstrated that PA treatment prominently induced the apoptosis of hepatocytes.
Subtle inflammation of the LPV group in our study might therefore be induced by other pathways or by low-grade PARP activation under the detection level of the analysis method used.
Aliquots (10 μl) of unbound or bound fraction were analysed by SDS/PAGE followed by detection of PARP-1 by Western blot analysis using monoclonal anti-PARP-1 antibodies (clone C2-10; BD Pharmingen).
