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It should be noted that the high allocation power in our analysis might be due to the limited number of parent pairs, and that the assignment of a progeny to a larger parental stock might require a higher number of marker loci.
The chicken (Gallus domesticus) was domesticated in southern Asia at least 4,000 years ago from a parental stock of one or more species of jungle fowl (principally G. gallus).
Smaller populations on the other hand, which are isolated from the parental stock, are decoupled from the homogenizing effects of gene flow.
Figure 4 shows an example matrix including all possible crosses among the individuals of the parental stock.
Because of its higher genetic variability, the F0 parental stock should have priority over the F1s in any breeding scheme.
Analysis of the 42 parental stock specimens revealed 14 variable sites (1 transversion, 13 transitions), which defined 6 haplotypes (Table 1).
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In crosses 3 and 4, we hoped to enrich for dumpy vortex mutants by incorporating the e dpv) mutant [4] on chromosome 3 in the male and female parental stocks.
We found a significant reduction of lifespan in animals expressing either the dominant negative alpha transgene (Figure 2A, C) or the gamma RNAi element (Figure 2B, D) as compared to animals expressing a wild-type alpha sequence or to parental stocks.
Corazonin cell ablation consistently extended lifespan during these stresses in males and females, as compared to the parental stocks or w1118 genetic background controls, although the degree to which lifespan was extended varied with both gender and the nature of the stress (Figure 2).
Backcrossing to parental stocks may also obviate the immediate effects of outbreeding depression.
In addition, we set up control families with males collected from the parental stocks.
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