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Unlike most tree-codes, PKDGRAV3, uses a binary tree where parent cells are divided along the longest axis into two equal volumed child cells.
When using hierarchical cubic grids (instead of an adaptive tree), this is implemented by interacting only between non-neighbouring cells on the same grid level whose parent cells are neighbours (e.g. Cheng et al. [1999]).
The MCF7 parent cells are ER-α-positive and proliferate in response to estradiol.
According to this model, the mutant colonies that arise above the lawn of plated parent cells are actually initiated by partially revertant cells within the plated population.
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IRP2-transfectants and control parent cells were injected subcutaneously into the flanks of BALB/c (nu/nu) mice to form solid tumor xenografts (Fig. 1A).
The ratio of IC50 resistant cells vs IC50 parent cells was presented as the resistance factor (RF).
To establish this cell line, the MKN45 parent cells were continuously exposed to increasing concentrations (0.1 2 μM) of 5FU over a period of 1 year.
Ethoxyresorufin- O-deethylase activity levels, undetectable in MCF-7 parent cells, were low but readily measurable in MCF-7 Txt cells.
In contrast, the miRNA profiles from exosomes compared to their parent cells were less correlated (DKO-1 r = 0.67 0.81, DKs-8 r = 0.64 0.71, DLD-1 r = 0.64 0.69).
The transfectant and parent cells were stained with biotinylated Helix aspersa (HAA) lectin (Sigma-Aldrich, St . Louis MO, USA) at 4°C for 15 min.
Importantly, some miRNAs poorly expressed in parent cells were abundant in vesicles, suggesting a preferential release of a subset of miRNAs.
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