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Values obtained at sea level and at high altitude were compared by a parametric Students paired t-tests.
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Appropriate parametric (student's t-test) and non-parametric tests (Wilcoxon Rank Sum) were used for assessing continuous variables.
The parametric student T-test and the non-parametric Mann-Whitney U test were used to compare distributions between groups as appropriate.
Multiple comparisons tests were performed, where appropriate, using post hoc analyses for parametric (Student-Newman-Keuls test) or nonparametric data (Dunn's method).
Variables will be analysed with the parametric Student-t test or the non-parametric U-test of Mann-Whitney (Wilcoxon ranksumtest) and the Wilcoxon-test for two unpaired samples.
Data consolidated were expressed as mean±sem and p value was calculated using non-parametric student t-test.
The data violated assumptions of normality and homogeneity of variance (visual assessment of the frequency histogram and normal plot, Kolmogorov-Smirnov and Barlett tests), thus differences between treatments were assessed using a non-parametric Kruskal-Wallis (χ2) test (KW), followed by the non-parametric Student-Newman-Keuls (SNK) test to correct for multiple comparisons.
There was a statistically significant increase (none-parametric student's t-test) in target copies per ml in bone marrow samples when compared to blood samples.
There was an insignificant variation (p > 0.1 with none-parametric student's t-test) in Ct value when the PCR assay was performed on DNA extracted from whole blood or PBS inoculated with a known quantity of bacterial cells.
In order to compare quantitative variables among patients in each of the treatment groups, to explore associations between variables within each group, and to study the potential association between baseline characteristics and response, we used parametric tests (student's t-test or ANOVA) or their equivalent non-parametric tests (U Mann-Whitney, Kruskall Wallis) when normality did not hold.
A parametric unpaired student's test that compares the means of the two groups (day and night) was used to confront the number of arms protruding from the sediment during day and night time.
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