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In vitro and other parametric assays were performed in triplicate and results are shown as mean ± SD.
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The use of high throughput, multi-parametric assays will not only aid in the definition and diagnosis of complex human immune disorders affecting NK cell function but also advance NK cell biology through population-based assessment of molecular signaling.
This is further aided by availability of wide spectral range of fluorescent probes that has advantage of simultaneous multi-parametric assay.
Cell death characteristics were studied using a multi-parametric HCS assay described in detail previously [ 23].
Results revealed the weakness of NOEC and LOEC notions, confirmed the feasibility of the proposed alternatives and allowed to discuss the – often violated – conditions that minimize the CI of the parametric estimates from DR assays.
To further evaluate this possibility, we conducted multi-parametric cytometry assays of major immune cell populations including neutrophils, dendritic cells, CD4/CD8 T cells and Treg cells present in small intestines of SHIP-deficient and WT mice.
The interactions of 4-methylenesterols with ERRs were investigated through a multi-parametric approach involving biological assays and molecular modelling.
Using the high throughput Complex Object Parametric Analyzer and Sorter (COPAS) assay and microscopy, we also found that the hlyA gene causes growth retardation in C. elegans.
Data obtained from dense granule secretion, clot retraction, spreading and cyclic nucleotide assays were analysed using parametric repeated measures anova.
Statistical analysis was carried out using MS excel and SPSS including Chi-square test and non parametric Mann-Whitney-U or Kruskal-Wallis assay.
The data obtained from aggregation, fibrinogen binding, α-granule secretion and in vitro thrombus formation assays were analysed using non-parametric repeated measures anova (Friedman test).
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