Equations to predict 17β-estradiol and 2,4-D sorption and mineralization parameters were established using Partial Least Squares regression.
Gating parameters were established using negative controls.
Optimal K-mer size and K-mer coverage parameters were established using a simple grid search (values of K from 21 to 81 bp) coupled with careful examination of K-mer frequency plots.
Relationships among significant parameters and each quality were established using the response surface methodology (RS M.
Distance trees of the nucleotide sequence data were established using the following parameters: i) 572 characters, ii) distance measure = LogDet/paralinear, iii) ties were broken at random, iv) 1000 bootstrap replicates were used for statistical analysis.
Gene expression profiles were established using the same parameters as in WT1 knockdown experiments.
The decision models were established using the surf similarity parameter, slope angle (cot α), beach permeability factor (Sp), relative wave height (H/h), wave spectrum (Ss) and wave momentum flux (m).
Effect of temperature, treatment time, rutin concentration and the atmosphere used for establishing the pressure in the reactor was studied and the optimal combination of reaction parameters was established using response surface methodology in a 3 × 3 Box-Behnken design.
The maximum likelihood phylogenetic analysis of the amino acid sequence was established using the following parameters: i) 290 characters, ii) 229 site patterns, iii) 13 constant site patterns, iv) model of substitution = JTT, v) 1663740 quartets were analysed with 146464 unresolved quartets, vi) 1000 puzzling steps were used for statistical analysis.
Calibration curve for the TIMP-1 immunoassay was established using the 5-parameter nonlinear regression model in Bio-Plex Manager™ 6.0.
