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Several parameters were assayed to optimize the procedure of the previous VFAE protocol (Gomaa et al. 2017).
The serum biochemical parameters were assayed to further evaluate the toxicity of nano-anatase TiO2on the liver of mice.
But not all the parameters were assayed in each sample.
All biochemical parameters were assayed on an Olympus AU400 autoanalyzer.
Biochemical parameters were assayed according to standard methods.
For the 20% liver homogenates the same parameters were assayed only the creatinine was replaced by the glutathione [ 23].
Similar(48)
At least two samples per sort parameter were assayed using an ABI7900 real-time PCR machine (Applied Biosystems).
The issue of "protocol drift" is serious because as protocols change--usually because someone in the lab thinks a modification will save time--the parameter being assayed may also change.
For all samples, each evaluated parameter was assayed in duplicate.
For all parameters, the samples were assayed in duplicate and all determinations were made simultaneously.
The kinetic parameters of Spr Cel8A were assayed under optimal pH and temperature conditions with CMC as the substrate, under different concentrations ranging from 0.2 to 1.2 mg/mL.
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