The histological grading system is constructed from a parameter of differentiation (glandular formation), nuclear appearance and a clear proliferation parameter (mitotic count).
The 4-parameter Hill-Slope curve fittings were generated by GraphPad Prism software using the least squares fitting method, and EC50 values were calculated for the three major parameters of oligodendrocyte differentiation: number of differentiated oligodendrocytes, number of oligodendrocyte processes, and oligodendrocytes processes length (µm).
When 0.1 μM ddC was supplied for 10 days before the differentiation protocol, the parameters of adipogenic differentiation were no different to those of untreated cells.
Experiments employing this design have led to the following findings: while cyclin A/E-CDK2 activity correlate with cell population growth parameters independently of differentiation status of cells, differences in cell fate are associated with existence of several types of molecular complex formed by D-type cyclins, CDK4, or CDK6 and p27.
The present study is designed to determine the effects of neonatal and prepubertal exposure to BBP on rat mammary gland at different stages of development using morphological parameters of gland differentiation [ 13, 14], proliferative index and the genomic expression profile.
Then, we have used morphological parameters of gland differentiation [ 16, 17], proliferative index, and genomic signature as biomarkers for detecting how prenatal exposure affects the post-natal development of the mammary gland.
By using the same co-culture system, we investigated whether myeloma cells could impair, through sclerostin production, specific parameters of OB differentiation such as production of extracellular bone matrix proteins.
Therefore, we used a computer simulation approach within the Approximate Bayesian Computation (ABC) framework [ 42] to estimate several parameters of population differentiation under alternative scenarios for the peopling history of the West Mediterranean region, and to estimate a selection coefficient for HLA-DRB1.
In this series there was no significant correlation between clinical parameters (ascites, degree of differentiation, residual tumor, tumor type or age) and survival.
