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For this purpose, we used the MAQ software [86] with default parameters for mapping the sequenced reads onto the Staphylococcus aureus strain N315 [34], considering both, the genomic and plasmid sequences (RefSeq NC_2745 and NC_3140).
We used default parameters for mapping with BMap.
Tophat2 (version 2.0.11) [ 38] was used with default parameters for mapping and reads with multiple alignments were discarded prior to counting gene expression, whereby only uniquely mapped reads were used for all subsequent analyses.
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A December 2006 memorandum of understanding mapped out rough parameters for the conception of such a park.
Default parameters for map-order optimization were used except that chain length was increased from 10,000 to 20,000.
Parameters for map construction were a minimum logarithm of odds (LOD) score of 3.0 and a maximum recombination fraction of 0.4.
These results, which demonstrate the strong influence of tonal amplitude on activation levels, identify sound intensity as an important adjunct parameter for mapping the functional architecture of auditory cortex.
The extent of LD is an important parameter for mapping genotype-phenotype associations within a population.
We used default parameters for the mapping and then filtered the mapped reads by mapping quality score [ 57].
Other factors that may contribute to the low mapping rates include the strict parameters used for mapping.
This was achieved by either using BWA mem version 0.7.4 (ref. 56) with default parameters for read mapping, followed by scaffolding individual BACs using SSPACE version 3.0 Standard57, or with SOAPaligner/soap2 version 2.21 and using SOAPdenovo54 scaffolder version 2.01.
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