Note that in particular cases, some biological restrictions applied to k values during optimization enable the proposed method to constrain the range of values permitted for some unknown parameters which are otherwise not determinable.
This study found that, by combining the time course of tumour volumes with a measure of Ki67, the estimates of parameter values were precise enough to permit a potentially useful and informative classification of the responses.
While justifications in terms of the literature are perfectly valid, and point estimates of the goodness-of-fit to experimental data for some specific combination of parameter values can provide insights and permit model comparison [ 8], we propose a quantitative measure of evidence derived from the fit of the model to the data.
Both task allocation processes are also robust to parameter variation permitting intuitive selection of parameter values.
This model is validated using data for delivery of a green fluorescent protein (GFP -encoding plasmid by means of Lipofectamine, permittinGFP -encodingon of model plasmider values.
In the method, the value of a single parameter was randomly selected from a restricted range of values while all other parameter values were randomly selected from the full range permitted by the baseline parameter space.
The probabilistic analysis, based on Monte Carlo simulation techniques, ran the model over a large number of times (n=1,000) with parameter values randomly drawn according to their distribution 15 to permit exploration of parameter uncertainty.
It's conceivable that life could exist in a universe with parameter values that we do not typically believe are life-permitting.
However, the model framework developed here is extremely flexible and permits interrogation of different forms for any of the parameter values and constitutive laws presented.
Parameter values that are not set or that are incorrectly set (e.g., outside the range of permitted values) will generate error messages, and visual indicators will highlight the problem parameter(s) so they can be corrected.
