The samples were named as follows: sample PL, where HTT was applied parallel to the sample surface; sample PP, where HTT was applied perpendicular to the surface; sample NF (no-field) annealed without magnetic field; and as-grown sample is named sample AG.
PCR and extraction blank reactions were carried out in parallel to the sample PCRs.
Parallel to the sample analysis, a set of certified sample material (CRM 278 Community Bureau of Reference, Commission of the European Communities) was also analysed to ensure reliability.
This can be achieved by the modification of the standard procedure to perform the trap cleaning in parallel to the sample conditioning, before extraction of the next sample begins (see ESM Fig. S 7).
Consequently it is possible to detect lattice planes that are parallel to the sample surface.
Consequently it is possible to detect lattice planes which are parallel to the sample surface.
During the analyses, blank samples were run parallel to the samples to control for possible contamination in the laboratory, and no such contamination could be identified.
Standards at a series of concentrations were run parallel to the samples.
Molecular mass markers were purchased from Bangalore Genei (India) and run parallel to the samples.
In each analytical batch, one reagent blank (water instead of plasma), one serum blank, the calibration standards [all standard substances purchased from Sigma-Aldrich (Taufkirchen, Germany), concentration of the analytes 2.5 to 50 μg/L, prepared in bovine serum], and one quality control sample were analyzed parallel to the human plasma samples.
To exclude a possible re-infection, a second serum sample parallel to the first sample was tested 4 weeks later.
