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Enucleated eyes were placed in custom-designed methacrylate eye-holders mounted on a micro-positioning stage in front of the microscope (with the optical axis parallel to the optical table).
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To achieve this goal, equal amounts of astigmatism was generated along two perpendicular planes, parallel and perpendicular to the optical table [ 3, 36].
Birefringence is the difference between the refractive index parallel to the optical axis of the crystal and the refractive index perpendicular to the optical axis.
As it passes through a liquid crystal, light polarized parallel to the optical axis travels at a different speed than light polarized perpendicular to it.
In addition, we will always guide two UV hollow-core fibers in parallel to the optical bench.
Let us denote the component of the dielectric tensor which is normal (parallel) to the optical axis of the crystal as ε ⊥.
For lenses made with spherical surfaces, rays which are parallel to the optical axis, but at different distances from the optical axis, fail to converge at the same point.
Following the imaging studies all specimens were formalin fixed and the tissue blocks were sectioned parallel to the optical imaging plane.
It was assumed that wall motions were parallel to the optical axis (i.e. Doppler angle of 0°).
We define the center of the scan as the unique position where light is reflected from a tangent plane that contains the coverslip-buffer interface such that the optical path will be parallel to the optical (z) axis (Fig. 3).
The Nikon Epi-fluorescence condenser was replaced with a custom condenser in which laser light was introduced into the illumination pathway directly from the output of a 3.5 µm optical fibre oriented parallel to the optical axis of the microscope.
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