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In the case of TPP, the molecules are preferentially oriented with the porphyrin ring parallel to the gold surface [37].
On polycrystalline gold, the POBA film consisted of lamellae standing with the c-axis parallel to the gold surface.
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Briefly, thiolated-PEG-biotin was conjugated to the gold surface.
Since the released MB has more chances to approach the gold surface, the MB signal increases.
Similar to the results obtained in the case of probe-DNA attachment directly to the gold surfaces, no significant changes in R ct values are observed between the bare gold surfaces and the monolayer-coated gold surfaces.
Specifically, Fig. 3a illustrates the superimposed CV responses of bare gold surfaces after mixed monolayer formation and probe-DNA attachment directly to the gold surfaces.
The annealed DNA was then incubated on the gold surface to enable attachment.
The formation of gold thiolates leads to a stable TSHP monolayer on the gold surface.
The stamps were then placed on the surface of the Piranha-treated gold, left for 15 seconds and then the gold surface allowed to dry for 10 minutes.
Reference spectra were acquired on the gold surface adjacent to the adhesive.
Putting the four DNA nucleosides in the middle of gold [111] nanoelectrodes with base planes parallel to the electrode surface layer, we study the transverse electronic transport properties of four nucleosides along the direction of electrodes.
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