In addition, parallel tagged sequencing [6] using sample-specific barcoding adaptors with 5'-nucleotide tagged PCR primers [34] would enable the analysis of multiple samples in a single sequencing region.
Meyer, M., Stenzel, U. & Hofreiter, M. Parallel tagged sequencing on the 454 platform.
Wielstra, B. et al. Parallel tagged amplicon sequencing of transcriptome‐based genetic markers for Triturus newts with the Ion Torrent next‐generation sequencing platform.
Using the parallel tagged sequencing method described by Meyer et al. [ 57], palindromic 20-bp DNAs, whose sequences differ from species to species, were ligated to both 5' and 3' ends of the repaired products.
Stoeck, T. et al. Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water.
Most recently, massively parallel tag sequencing techniques [71] have revealed that, like other oceans, the Arctic contains a remarkably diverse range of microbes [65].
Massively parallel, tag-based sequencing systems, such as the SOLiD system, hold the promise of revolutionizing the study of whole genome gene expression due to the number of data points that can be generated in a simple and cost-effective manner.
This will help interpreting the vast diversity of tags generated by massively parallel tag sequencing.
Massively parallel tag sequencing (454 sequencing, pyrosequencing) is a promising remedy and offers a means to more extensively sample molecular diversity in microbial communities [ 13].
We applied a high-throughput parallel tag sequencing (454 sequencing) protocol adopted for eukaryotes to investigate protistan community complexity in two contrasting anoxic marine ecosystems (Framvaren Fjord, Norway Cariacoo deep-sea basin, Venezuela).
