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To determine which of the two PPREs within the human NHE1 is the true PPARγ binding site, we transfected MCF-7 breast cancer cells with a full length NHE1 promoter construct (containing both PPRE1 and PPRE2) compared to a parallel set up transfected with a 5' deletion of the full length (only contains PPRE2).
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That parallel sets up some really meta moments in the premiere, like when Dan Conner John Goodmann) comforts his daughter Darlene (Sara Gilbert) saying, "[Roseanne] was going to do what she was going to do.
"This is a parallel track set up by enemies of Syria to compete with Kofi Annan's mission, maybe to undermine his mission".
Parallel wells set up without heme are used to correct for A415 absorbance by compounds.
Instead of an assault through the roadside bombs, six battalions would drive in on parallel streets and set up checkpoints and search for weapons.
Of course, all strategies need to be considered in light of the alternative minimum tax, a parallel tax system set up in 1969 to ensure that the wealthiest taxpayers paid their fair share of taxes.
Aid agencies also want a parallel agreement to set up registers of who owns and controls anonymous "shell companies" to be made public rather than limiting access to them to tax authorities.
In the absence of an honest and complete report, observers may have to look to the findings of an "Alternative Truth Commission," which could be similar to parallel inquiries previously set up in other Latin American countries.
The AIF was administered separately from the home-based army in Australia, and a parallel system was set up to deal with non-operational matters including record-keeping, finance, ordnance, personnel, quartermaster and other issues.
In parallel we also set up a technical replicate in which the dyes were switched/swapped between each set of 24 and 66 h samples to overcome bias due to the dye itself [64].
A set of parallel reactions was set up, each substituting single UUU or UUC (Phe) codons with NBR and NVN libraries (where N = A, C, G, or T; B = C, G, or T; R = A or G; V = A, C, or G), hence encoding all canonical amino acids except phenylalanine.
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