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In conclusion, we have shown that the Fmoc-SPPS of Ub reported here enables the parallel incorporation of desired tags, labels, and mutations in high yields with high purities.
SNARE-based protein stapling does not result in the formation of the peptide bond between the two parts, but allows parallel incorporation of chemical agents, e.g., fluorophores or other labeling compounds.
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In parallel, PI incorporation was observed to assess membrane function.
Although Rothberg et al. demonstrated a FET-based DNA sequencing system utilizing massively parallel nucleotide incorporation detection with millions of reaction wells using a single reference electrode, this assay is limited to reactions that occur in pulses through sequential addition of nucleotides.
Controls without exogenous acceptors were run in parallel and their incorporation was subtracted.
Cell numbers of non transfected cells have been quantified once in parallel by thymidine incorporation assay.
In some experiments, we used rat subcutaneous fibroblasts obtained using the method described before to monitor single-cell [Ca2+]i oscillations (see above) in parallel with the incorporation of FM4-64 (Molecular Probes, Invitrogen), which is a membrane-selective fluorescent dye used to evaluate vesicle endocytosis and exocytosis in living cells [ 33, 34].
According to the phylogenetic tree of the family, the cytosolic form block (LSG1) is distinct from the nuclear form blocks (NOG2, NGP1, YawG), which later expanded into a nucleolar form (NUG1), in parallel with the incorporation of members upon engulfment of the future mitochondria (MTG1) that cluster within the YlqF branch as well as the future chloroplast (ChYlqF).
First, since PKA and CaMKII act in parallel in controlling synaptic incorporation of GluR1 [39], reduced phosphorylation of GluR1p845 may affect activity-dependent AMPA receptor trafficking.
Paraformaldehyde treatment did not significantly affect the cellular uptake of [C]oleate as indicated by parallel measurements of the incorporation of [C]oleate into [C]triglycerides.
The potential non-linearity of ipilimumab PK was assessed during base model development by incorporation of parallel linear and non-linear elimination.
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