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We show the relevant programming paradigms that allow multi-level parallel expression of these methods and how the software engineering technology can contribute significantly in achieving high scalability.
Here, we describe the design and use of a set of vectors developed around a unified cloning strategy that allow parallel expression of target proteins in the baculovirus system as N-terminal or C-terminal fusions.
These functional data were accompanied by a parallel expression of both CAMs in the mesenteric microvasculature.
In parallel, expression of eIF4ES, and its mutant derivatives, was used to complement translation in an eIF4E-deficient yeast strain [47].
In parallel, expression of the transcription factor ATF4 is selectively enhanced along with the expression of downstream target genes such as GADD34, CHOP/GADD153 and others, which participate in the control of cellular redox status and cell death [12].
The establishment of rapid cloning, expression and protein detection procedures has therefore become a major field of interest for the design of high-throughput methods for parallel expression of proteins in multiple expression systems.
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Indeed, under the influence of 19th-century German idealism and parallel expressions of rising European nationalism, there were some the Marxists, for example—who, though not rejecting individual rights altogether, maintained that rights, from whatever source derived, belong to communities or whole societies and nations preeminently.
The bi-cistronic expression of the transgene and the drug resistance gene using an internal ribosome entry site (IRES) is able to confer a tight correlation between the transgene expression and the drug resistance because the IRES-mediated cap-independent translation ensures parallel expressions of the transgene and the drug resistance gene [ 8].
Although high-throughput protein expression has been described for e.g. E. coli [12], [13], [14], [15], insect cells [16], mammalian cells [17], [18] and for in vitro systems [19], rapid and cheap detection of recombinantly expressed proteins is still a time-consuming factor and remains a major bottleneck for multi-parallel expression of large numbers of different proteins.
We have shown that autoimmune MRL-lpr/lpr mice overexpress NOS2 and overproduce.NO in an age-dependent fashion that parallels expression of arthritis, glomerulonephritis, and vasculitis.
The method is applicable to several cell-types and can be scaled for parallel expression measurements of hundreds of gene promoters.
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