The establishment of rapid cloning, expression and protein detection procedures has therefore become a major field of interest for the design of high-throughput methods for parallel expression of proteins in multiple expression systems.
The CD133 positive subpopulations within the xenograft panel were further analysed for parallel expression of CD44 and CD24 or CDCP1.
It has been demonstrated that P. pastoris is an efficient production system also for very large and complex proteins, such as collagens, which besides the recombinant gene(s) needed for the collagen polypeptide chain(s) needs the parallel expression of two different genes coding for collagen prolyl 4-hydroxylase (C-P4H), an enzyme required for the thermal stability of collagens [ 7- 9].
Specifically, we performed parallel expression analyses for MC1R mRNAs in the two lizard population (n = 5 males and 5 females for each population, see Additional files for full details).
We developed a method for parallel expression and purification of recombinant proteins with a hexahistidine tag (His-tag) or glutathione S-transferase (GST -tag from bacterial expression systems.
The method is applicable to several cell-types and can be scaled for parallel expression measurements of hundreds of gene promoters.
Although high-throughput protein expression has been described for e.g. E. coli [12], [13], [14], [15], insect cells [16], mammalian cells [17], [18] and for in vitro systems [19], rapid and cheap detection of recombinantly expressed proteins is still a time-consuming factor and remains a major bottleneck for multi-parallel expression of large numbers of different proteins.
Investigation of the chromosomal location of these miRNAs confirmed a reasonable explanation for the parallel expression - they are encoded in the same transcript [ 9, 18].
These considerations motivated the proposal, in the 2005 version of this article, that mou referred very generally to taking any two parallel expressions, "proceeding" to assert a further expression on the basis of one of them, and then using that as grounds for asserting a parallel expression on the basis of the other.
This feature could be an enhancer or an LCR common to both genes and responsible for their observed parallel expression profiles.
