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Briefly, the immunofluorescence quantification was performed using the formula: where L and R denote proximal and distal positions respectively in a parallel division and as left and right cell respectively in perpendicular division, to get the normalized ratio for PH3 and β-Catenin.
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How are differential fates conferred to the daughters arising from parallel divisions?
However, the number of parallel divisions increased gradually between P5 P14 (Fig. 1D,E).
In the SAG treated animals, the percentage of parallel divisions was significantly reduced (46%) (Fig. 2R).
In Drosophila embryos, Insc acts as a switch to drive cells toward vertical divisions, whereas cells with inactive Insc drive parallel divisions [ 37].
Blocking of Shh signalling led to a sharp decrease in the number of perpendicular divisions and a corresponding increase in parallel divisions.
Interestingly, in Cyclopamine treated animals, there was a dramatic increase in the percentage of parallel divisions (71%), when compared to control (56%).
The increase in parallel divisions at later ages observed in the current study correlates with the period of decrease in GNP proliferation during later development.
For parallel divisions, positive values denote that the cell proximal to the pial surface has more β-Catenin compared to the cell distal to the pial surface.
The percentage of parallel divisions was calculated for different animals and weighted mean and variance for the percentages for each age was determined.
This debate within the left also parallels divisions evident in earlier wars.
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