Sentence examples for paraffin kit from inspiring English sources
Total RNA was isolated from cells using Trizol (Invitrogen, Carlsbad, CA, USA) and from paraffinized tissues using the High Pure RNA Paraffin Kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer's instructions.
RNA from FFPE material was extracted using the High Pure RNA paraffin kit (Roche), which is the method recommended by Illumina for DASL applications [8], [9].
RNA extraction was performed using a commercial kit (High Pure RNA Paraffin kit, Roche).
Total RNA was isolated using High-Pure RNA Paraffin kit (Roche Diagnostics, Penzberg, Germany).
For FFPE samples, total RNA was isolated using Roche High Pure RNA paraffin kit, Cat# 03270289001.
The High Pure RNA Paraffin Kit (Roche Applied Science, Mannheim, Germany) was used to isolate the RNA from the paraffin.
Air-dried tissue sections were treated with a Paraffin Pretreatment Kit (Vysis Inc).
Paraffin-embedded LCLC sections (4 µm) were baked, deparaffined by washing in xylene, and dehydrated in 100% ethanol, and then incubated in pretreatment liquid (paraffin pretreatment kit) for 20 to 25 min at 80°C.
Sections of 4 μM were mounted on glass slides using a PSA® Paraffin Sectioning Kit (Alphelis, Plaisir, France), and subsequently stained according to standard immunohistochemical procedures using citrate antigen retrieval.
RNA was extracted from these tumour cores using the protocol in the High Pure Paraffin RNA Kit (Roche Diagnostics Ltd ,Burges Hill, UK) and supplied to a service provider for gene expression studies using the Illumina DASL Cancer Panel, which targets 502 cancer genes with unique probes in three different locations per gene (Illumina, 2005b).
The 6 μm thick tissue sections on slides were deparaffinized in xylene, followed by dehydration with absolute ethanol, air-dried and pre-treated using the Vysis Paraffin Pretreatment Kit I (Vysis, Inc .. Following pre-treatment, the hybridization process was performed at 37°C for 14 18 hours (overnight) and the nuclei were then counterstained with 4'-6'-diamidino-2'-phenylindole (DAPI).
