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After blotting, nitrocellulose papers were incubated with specific antibodies.
After the blotting, nitrocellulose papers were incubated with specific antibodies.
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The solution was removed and the filter paper was incubated with 1 ml PBS at 4°C for 30 minutes.
1 cm2 of the filter paper was incubated overnight in 1 ml PBS (Sigma) +0.5% saponin (Sigma) at 4°C.
After the removal of PBS, the filter paper was incubated at 100°C with pre-heated 200 µl PBS +5% Chelex-100 (BioRad™) for 10 minutes and vortexed vigorously every 5 minutes.
The gel slice along with the 3 mm paper was incubated in 100 μL deionized water for 30 min, boiled for 15 min in a tightly capped eppendorf tube, and the gel materials were pelleted by centrifugation.
The paper disks were incubated for 3 min in liquid nitrogen, thawed and soaked with Z buffer (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM MgCl2, 50 mM 2-mercaptoethanol, pH 7.0) containing 1 mg.mL−1 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal).
Overnight broth cultures (10 μl) of both the donor and recipient bacteria were spotted together onto a filter paper and were incubated overnight at 37 °C.
After addition of recombinant protein discs papers, the plates were incubated at 37°C overnight and the clear zone diameter was visually monitored as the inhibition of bacterial growth.
Whatman chromatography no 2 filter paper was used to prepare 6.3 mm paper discs that were incubated with 20 μL aliquots of the re-dissolved extracts from lyophilized decoctions or juices at their final concentration and at 25%and50%0% dilutions, and air-dried for 2 hours, as described previously [ 13].
Paper rolls with seedlings were incubated in aerated PEG solutions or distilled water for 6 h and 24 h (24°C, 24 h included 8 h darkness, 18°C).
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