Exact(1)
Statistical comparisons in data-containing panels were performed using Student's unpaired t -test.
Similar(59)
Detailed analysis of the expression data obtained from these specific panels was performed using non-parametric Mann-Whitney test and confirmed HAX1 significant up-regulation in tumors, though not as high as previously detected in the survey panel (Fig 2).
(C) H&E staining (left panel) and Masson's staining (right panel) was performed using kidney sections.
Statistical analysis of the Illumina GoldenGate panel was performed using the Mann–Whitney test as calculated by GraphPad prism.
The endocrine hormone panel was performed using the commercially available Milliplex Mouse Metabolic Hormone Magnetic Bead Panel MMHMAG-44K (EMD Millipore Corporation, Billerica, MA) and Luminex Multiplex Platform (MAGPIX®).
Library preparation for the 409-gene panel was performed using the Ion Torrent Ampliseq Comprehensive Cancer Panel Life Technologiess) following the manufacturer's instructions.
Sequence alignment and variant calling for the Fluidigm custom panel was performed using NextGene software v.2.3.4 (SoftGenetics LLC, State College, PA, USA) using defined parameters that included a minimum read depth of 500x and nonreference allele frequency of at least 5%.
To explore the potential for CTCs to serve as a source of tumor DNA for genomic profiling in HCC, next-generation sequencing using a targeted cancer gene panel was performed using whole genome-amplified DNA derived from pooled purified CTCs, along with DNA from paired archival, paraffin-embedded tumor tissue and peripheral blood mononuclear cells when available.
Post-acquisition enhancement (i.e. 'normalization' or altering the range of pixel intensity values for viewing purposes), MIP and panel combination were performed using ImageJ.
A complete blood count, chemistry panel and a urinalysis were performed using standard laboratory techniques.
All regressions were performed using panel data with year indicator variables.
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