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Immunofluorescent staining was performed for GLUT-1 (green) and nuclei (blue) on all panels of cells from Figure 1, which had been treated with drug for 3 days.
The extent of the contribution of checkpoint weakening to tumor formation is thus controversial and conclusions will await examination of chromosome segregation in large panels of cells from patient tumors.
Similarly, no significant association was observed for panels of cells other than T lymphocytes.
We tested whether the disease-associated STAT4 SNPs would alter expression of the alternatively spliced STAT4β isoform by utilizing expression data from Affymetrix Exon 1.0 arrays in two population-based panels of cells of both lymphoid (lymphoblast) and mesenchymal (osteoblast) origin (19).
Figure 6b d shows records of APs obtained at a stimulation frequency of 1 Hz under conditions of normal extracellular pH (left panels) and after 5 min of subsequent metabolic acidification (right panels) of cells patch-clamped using either the BAPTA (Fig. 6b,e) or the EGTA-BAPTA solution (Fig. 6c,d).
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AstraZeneca hopes to establish panels of cell lines and potential biomarkers, as well as in vitro assays to support translational research.
This also holds true for other panels of cell lines [38], and data not shown.
Our algorithm enables the assembly of panels of cell lines representative of the genomic heterogeneity of cancers.
The mutant VHL expression constructs were used to generate several panels of cell lines that are described throughout this report.
A major difference compared with previous screens is the evaluation of heterogeneous panels of cell lines rather than engineered isogenic cell lines.
We then examined CAML protein levels in the same panel of cells by Western blot.
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