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†, panel strain (12 ); suprascript T, type strain for species.
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In the bottom panel, strains were cultured in YDP (Y) or CSM (-C).
(B ) In the top panel strains were grown in glucose or for 1 hr in galactose (GAL, +).
Apobec1 expression varied significantly among the hybrid mouse diversity panel strains and the major locus regulating expression mapped directly over the Apobec1 gene, at 122 Mb on Chromosome 6 (data not shown).
Comparing expression within the isogenic strain panel allows us to identify genes whose expression does not increase linearly as gene copy increases; comparing expression in the panel strains to the closely related euploid reference identifies heritable expression differences in the two strain backgrounds.
MATS provides an estimate of vaccine coverage against a panel of strain isolates from a given region.
Percentages achieving hSBA titers ≥4 against these heterologous strains were lower than the indicator strains, but killing was evident across a broad panel of strains as early as 5 months of age.
In fact, both of these elements were more prevalent among the panel of strains of N. farcinica than N. cyriacigeorgica strains selected for this study.
55 In addition, the MATS assay is based on a limited panel of strains which may not be directly applicable to the diverse strains causing disease in the UK.
At 5 months of age, following the second dose at 4 months, although percentages achieving hSBA titers ≥4 against reference strains were lower, killing was already evident across a broad panel of strains.
The pCS2 probe did not hybridise to the genomic DNA from any of the remaining C. sordellii isolates in the panel of strains tested indicating that this plasmid is not present among these strains.
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