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Because of limited volumes of sera and restrictions in shipping of biological specimens internationally, each evaluation center assembled its own panel following proficiency testing and sample revalidation using study-specific standard operating procedures and materials.
19 The analysis has been validated as proficient using the World Health Organization HPV DNA genotyping proficiency panel.
The laboratory as well as those participating in the small proficiency panel described here—has participated in all proficiency panels on HLA tetramer analysis of the CIMT immunoguiding program (CIP; http://cimt.eu/workgroups/cip/proficiency-panel-program/).
Obviously, all commonly available freezing media could not be tested in a single proficiency panel, and the selection of three media used in the proficiency panel was made by the participating centers which lead to the fact that a freezing media containing FCS, which is probably the most common supplement used was not included in the proficiency panel.
Two factors that might have contributed to this detection rate "above average" are that (1) the five CRI-CIC laboratories that participated in this study were known to be top performers in former proficiency panels and (2) 14 laboratories in this panel already participated in previous ELISPOT proficiency panels of CIP.
A proficiency panel was designed to assess the performance of nucleic acid amplification technologies for the detection of Toxoplasma gondii in amniotic fluid.
An HSV-2 proficiency panel was developed at the University of Washington and implemented through Contract Laboratory Services (CLS, Dr. Wendy Stevens, Johannesburg, South Africa).
However both readers passed the initial proficiency panel on the first occasion, and had similar performance in intra and inter-reader variability (Reader 2 had slightly lower rate of errors).
*last 3 months of proficiency panel results (November - January 2011).
Figure 6 depicts the results from this proficiency panel.
We performed a small proficiency panel with our standard samples.
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