We developed a panel for selective enrichment of a set of 56 genes known to play a role in monogenic diabetes followed by next generation sequencing (NGS) using the Illumina MiSeq platform.
Using a panel of isoform-selective inhibitors, we show that insulin signalling to Akt/PKB (protein kinase B) is attenuated by the additive effects of inhibiting p110α/p110β/p110δ in all cell lines tested.
To fully characterize how distinct inactive ATP-binding site conformations affect the regulatory domains of SFKs, we assembled a panel of conformation-selective inhibitors that stabilize both the αC helix-out and DFG-out forms.
Given the surprising differences that were observed in how regulatory domain engagement affects ATP-binding site conformation in Fyn1 and Fyn2, we next investigated how αC helix conformation affects intramolecular regulatory domain engagement using our panel of conformation-selective ligands.
Using a panel of conformation-selective, ATP-competitive inhibitors in combination with a panel of Fyn1, Fyn2, and Lyn regulatory state mutants, our study explores the degree of allosteric coupling between each regulatory domain and the ATP-binding site for each SFK.
These studies were conducted with a panel of three DNA-selective enediyne antibiotics (calicheamicin γ1I, esperamicin A1 and neocarzinostatin) that produce different proportions of double- and single-strand deoxyribose damage in DNA, with genome-wide responses compared to those induced by the non-selective γ-radiation.
In conclusion, we developed a differential SELEX-based procedure that allowed us to generate a highly informative panel of few specific and selective aptamers for malignant glioma cells.
Comparatively low isolation rates for S. aureus and/or high isolation rates for S. pyogenes from RT patients as described in some studies [10], [13], [17], [18], [44] could be due to geographical or seasonal effects [25], a selective panel of detection methods, or to temporal variations as described by Timon et al. [20].
Seven core molecular classes were identified using a selective panel of 10 biomarkers.
